Pesticide residue is a significant problem in agriculture that greatly affects human health and life safety.Therefore,it is an urgent need to develop simple and effective method for the rapid detection of trace pesticide residue.In this work,combined with a portable Raman spectrometer,a surface-enhanced Raman spectroscopy (SERS)method was developed to achieve the rapid detection of trace organophosphorus pesticides including fenthion and parathion using gold sols as the SERS substrate.It was found that the characteristic Raman peaks of fenthion and parathion located at 1053 cm-1,1216 cm-1 and 857 cm-1,1112 cm-1,respectively,thus enabling the multi-target analysis simultaneously.The concentrations of fenthion and parathion display a linear relationship with their corresponding Raman intensities with a detection limit of fenthion can reach 0.01 μg/mL and parathion can reach 0.025 μg/mL.Furthermore,the SERS method developed here was successfully applied in the direct detection of fenthion and parathion residue on practical spinach samples with a detection limit of 0.05 μg/mL.This work demonstrates that SERS can be a convenient,rapid,sensitive and multi-target analysis method for the on-site detection of pesticide residues.