He-Ne laser irradiation combined with salvia miltiorrhiza injection (SM) can treat ischemia and anoxemia well in clinic, but the mechanism remains obscure. In this paper, the effects of low intensity He-Ne laser irradiation, SM, and the combination of He-Ne laser irradiation and SM on the respiratory burst of bovine neutrophils were studied, respectively, which may help to understand the mechanism of the combination of He-Ne laser irradiation and SM fight against ischemic injury. The chemiluminescence was used to measure the respiratory burst of neutrophils. The results showed that 200 J/m2, 2000 J/m2 He-Ne laser irradiation can stimulate the respiratory burst of neutrophils, 40 J/2, 1000 J/m2, 6000 J/m2 He-Ne laser irradiation can inhibit the respiratory burst of neutrophils, 4～16 μL SM can inhibit the respiratory burst of neutrophils, and have the dose-effect relation. The inhibitory effect of the combination of 1000 J/m3 He-Ne laser irradiation and 8 μL SM is more marked than that of only laser or SM.