To investigate the clinical efficacy of 5-aminolaevulinic acid-photodynamic therapy (ALA-PDT) combined with liquid nitrogen cryotherapy in the treatment of recurrent condyloma acuminatum and its impact on local HPV6/11 viral load. 30 patients with recurrent condyloma acuminatum were treated by ALA-PDT combined with cryotherapy once every 7-14 days for 4 times. At the end of treatment, the patients were followed up every 2 weeks and the clinical efficacy was evaluated at 3 months after the last treatment. The real-time quantitative PCR was conducted to estimate the DNA loads of HPV6,11 in the specimens collected before the combined treatment and 2 weeks after the last treatment. HPV negative rate and the changes of viral load in patients with HPV persistent positive after combined treatment were analyzed. Of the 27 patients who completed the combined treatment, six patients (22.22%) recurred within 3 months after the last treatment. Among the 27 patients, 25 cases were HPV6/11DNA positive before the combined treatment, of which 16 cases (64.00%) turned negative 2 weeks after the last treatment. The changes of viral load in the other 9 cases of patients with HPV persistent positive before and after combined treatment were analyzed by 2-ΔΔCt relative quantitative method, which showed that the viral load of HPV6/11DNA in the primary site after combined treatment was significantly lower than that before treatment (S=-22.5，P<0.01). ALA-PDT combined with cryotherapy can effectively reduce the viral load of HPV6/11DNA in patients with recurrent CA, improve HPV negative conversion rate and reduce recurrence.