Rosmarinic acid, as a natural acid-secreting compound, has been widely used in the treatment of various cancers. In order to explore the therapeutic effects of rosmarinic acid on human cervical cancer. The experiments of cell proliferation, migration and autophagy were carried out in this study for functional verification, and Western blotting was used to verify the possible signaling pathways. The results showed that the proliferation ability of human cervical cancer significantly decreased after rosmarinic acid treatment; the cell migration ability was also inhibited, the expressions of autophagy-related proteins ATG5 and Beclin1 were up-regulated, and the expressions of PI3K, AKT and mTOR proteins decreased. This suggests that rosmarinic acid may promote cell autophagy and inhibit the proliferation and migration of cervical cancer cells by inhibiting the PI3K/AKT/mTOR signaling pathway, thereby achieving the effects of inhibiting or treating human cervical cancer. This further indicates the potential value of rosmarinic acid as an anticancer drug. It provides a new theory for the research and development of anticancer drugs.