As China's urbanization enters the stage of stock development, the update of the transportation system is shifting from scale expansion to a new stage of quality improvement. In this context, the transportation special physical examination, as a core technological path for improving infrastructure efficiency, has become an important lever for implementing the strategy of high-quality urban development. The current urban traffic physical examination mainly relies on urban physical examination, which has limitations such as one-dimensional and insufficient comprehensiveness. Taking the comprehensive transportation physical examination in Huzhou as an example, this study combines macro policy requirements and urban characteristics to construct a three-level physical examination evaluation system consisting of 5 dimensions and 32 indicators: "target dimension, evaluation index, and indicator system". Specifically, it includes five target dimensions: openness, efficiency, greenness, wisdom, and resilience, to quantitatively evaluate Huzhou's development level and existing problems in external transportation development, transportation operation efficiency, green travel, transportation emergency and intelligence.