Effects of glycine (Gly) and graphene oxide (GO) modified hydroxyapatite (Gly/GO/HA), and aspartic acid (Asp) and GO modified hydroxyapatite (Asp/GO/HA) on in vitro remineralization of acid-etched human enamel (HE) were investigated. The cytotoxicity of these composites was studied by CCK-8 method, the surface and cross-section morphology, surface element composition, surface composition structure and surface mechanical properties of HE before and after remineralization were characterized by field emission scanning electron microscope, EDX energy spectrum analysis, X-ray diffraction and microhardness test. The results show that Gly/GO/HA and Asp/GO/HA both have good safety and biocompatibility, and both can repair the acid-etched HE to a certain extent, among which Gly/GO/HA is better than Asp/GO/HA in repairing the surface and deep demineralization of the acid-etched HE.