Chinese Optics, Volume. 17, Issue 3, 512(2024)

Design of miniature head-mounted fluorescence microscope based on metalens

Zhi-miao ZHANG1,2, Cheng-miao WANG1, Mian XIE3, Yu LIN1, Ye-ming HAN1, Yong-bo DENG1, Chang-liang GUO4, and Qiang FU1、*
Author Affiliations
  • 1Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences , Changchun 130033 , China
  • 2University of Chinese Academy of Sciences, Beijing 100049, China
  • 3School of Life Sciences, Tsinghua University, Beijing 100084, China
  • 4College of Future Technology, Peking University, Beijing 100871, China
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    The recent advent of miniature head-mounted fluorescence microscopes has revolutionized brain science research, enabling real-time imaging of neural activity in the brains of free-moving animals. However, the pursuit of miniaturization and reduced weight often results in a limited field of view, constraining the number of neurons observable. While larger field-of-view systems exist, their increased weight can impede the natural behaviors of the subjects. Addressing these limitations, a novel design utilizing a metalens schematic is proposed. This approach offers the benefits of being ultra-light, ultra-thin, and capable of high-quality imaging. By deriving the aberration formula specific to hyperbolic phase metalens and using it as a foundation, a design for a miniature fluorescence microscope was developed. This microscope boasts a 4 mm×4 mm field of view and a numerical aperture (NA) of 0.14, effectively correcting seven primary aberrations. The resulting prototype, weighing a mere 4.11 g, achieves a resolution of 7.8 μm across the entire field of view. This performance is sufficient to image neural activity in the brains of freely moving mice with single-cell resolution.

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    Zhi-miao ZHANG, Cheng-miao WANG, Mian XIE, Yu LIN, Ye-ming HAN, Yong-bo DENG, Chang-liang GUO, Qiang FU. Design of miniature head-mounted fluorescence microscope based on metalens[J]. Chinese Optics, 2024, 17(3): 512

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    Paper Information

    Category: Original Article

    Received: Dec. 27, 2023

    Accepted: Feb. 6, 2024

    Published Online: Jul. 31, 2024

    The Author Email:

    DOI:10.37188/CO.2023-0237

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