The optical path structure of holographic storage with fiber coupling and wavelength multiplexing in the optical disk is detailed in this paper. Because of simple structure, less optical elements and higher ratio of capability to price, it will become a new core technology in the holographic optical disk drive. Because the performance of the short wavelength single mode optical fiber should be higher, a multi-model optical fiber is used to produce quasi-single mode output, and a beam prism employed instead of the optical fiber splitter. A photopolymer is made which is sensitive to two wavelengths as the storage material. Its sensitivity of spectrum is even, and the spectrum range is not overlapping. The experimental result indicates that the storage effect is better and the structure of optical path is reasonable.