In order to realize the identification of jadeite species and rubber-injected jadeite, theoretical analysis and experimental verification were carried out by using microscope combined with shifted-excitation Raman difference spectroscopy（SERDS), and SERDS data of 112 jadeite samples were obtained. The SERDS and ordinary Raman spectrum of jade were compared, and the Raman characteristic peaks of jade and the identification basis of B jade were statistically analyzed. The results show that the SERDS can effectively filter out the fluorescence and has a significant advantage in identifying the characteristic peaks of high-fluorescence jadeite samples. The peaks at 378cm-1, 698cm-1, and 1037cm-1 are relatively strong and have sharp peak shapes. They are the characteristic peaks with the highest frequency when detecting jadeite, which can be used to identify sample species. The cracks, pits and the organic matter filled by the pickling of the B jadeite can be observed by microscopic magnification. The Raman characteristic peaks of the organic matter can be detected here, of which 1114cm-1, 1188cm-1, 1611cm-1 appear most frequently, jade goods A and B can be identified through this method is simple to operate and the identification efficiency can be improved. This method can provide a reference for the rapid identification of jadeite.