A 800 nm femtosecond laser tweezer can be used to capture the silver nanoparticles dispersed in water and the silver wires are directly written on the glass substrate. The influences of laser power on the silver wire width and surface morphology are investigated by adjusting laser parameters. The direct writing of silver wires with a width of 378 nm is realized. After tests, the electrical resistivity of the fabricated silver wire is 19.88 times that of bulk silver. In addition, a two-dimensional (2D) silver grid structure is fabricated by this technique, which manifests that this technique possesses a good process ability in the field of 2D structure direct writing.