In the human-computer interaction scenario, in order to realize the hardware universal design of key matrix, function software custom settings, and the number of keys can be expanded, the OLED display keys are studied, and the key matrix based on OLED display keys is designed. According to the driving principle of OLED display keys, the circuit schematic diagram of key matrix based on LPC1788 controller is designed. The design scheme based on hardware generalization and the working principle of each part of the circuit are proposed. Then, according to the control sequence and state transformation of OLED display keys, the firmware design of LPC1788 controller and the configuration software design of the upper computer of OLED display keys are carried out, which realizes the configurability of the software for displaying text and pictures. Finally, the design of OLED display key matrix is tested, and the universal hardware settings, key function definable and extensible design are realized. The design and implementation show that OLED display keys can be designed as key matrix in human-computer interaction scene. The key display resolution is 96×64 pixels, 180° perspective, dimming range is 0~250 cd/m2, high definition, high contrast and full screen display. Through flexible configuration of software, matrix page turning design can be realized, which expands to more key demand and saves structure space. The OLED display key matrix design based on LPC1788 is stable, reliable, clear, versatile, beautiful in appearance, friendly in man-machine interface, and can be widely used in man-machine interaction system for keyboard requirements of vehicle, ship and airborne.