X-ray fluorescence spectrum(XRF)analytic method was used to analyze and compare the contents and varieties of mineral elements in the ground group Salvia miltiorrhiza Bge, and the fourth generation Salvia miltiorrhiza Bge with space flight mutagenesis breeding in order to provide important basic data for selecting high quality breed and further research. The result indicates that the varieties of the main mineral elements in the two samples are the same basically, but Cu/Zn ratio is enhanced to 1.0, the contents of microelements Cr, Mn and Fe increase by 0.6, 0.5, and 0.3 times respectively compared with the ground group. To conclude, in the space group the contents and proportions of the mineral elements are improved and optimized. X-ray fluorescence spectrum analytic method has many advantages, such as quickness, simplicity, high sensitivity, wide measure range, etc. The method can be used for quantitative element analysis and study of Chinese herbal medicines.