The seedlings of wheat (Jinmai No.8) are exposed to He-Ne laser irradiation (5 mW/mm2) and enhanced UV-B radiation [10.08 kJ/(m2·d)], the polyacrylamide gel electrophoresis (PAGE) is used to analyze the patterns of peroxidase (POD), catalase (CAT), esterase (EST), adenosine triphosphatase (ATPase), and malate dehydrogenase (MDH). The results have shown that two new bands of POD zymogram appeare under enhanced UV-B radiation, but the activities of the total POD isoenzymes decrease. However, there is no difference among isoenzyme bands of CAT, EST, ATPase, and MDH, but the activity of the isoenzymes decreases obviously. Our study find that the gene expression and activities of plant are weakened by UV-B radiation, but the ability of plant stress resistance is enhanced by He-Ne laser irradiation.