Currently, lasers are widely used in biomedical field for both diagnoses and treatment. At the same time, new laser techniques are investigated to improve the application efficiency. Due to its ultrashort pulse duration and high pulse intensity, femtosecond laser shows distinctive advantages over long pulse lasers. Since the characteristics of light propagation in ocular medium are basic concern for laser application in ophthalmology, in our research, light propagation in some ocular tissues was investigated. The samples were from fresh pig eyes. The laser source was femtosecond oscillator system at 800-nm wavelength. The linear and nonlinear phenomena during laser propagation in ocular medium were discussed. Without using chirped pulse amplification system, femtosecond oscillator system itself provides a low energy processing. Our experimental results suggest that the femtosecond oscillator system has a potential to ensure the precision of incision and minimize the collateral damage in glaucoma treatment.