In order to meet the optical instruments’ special demands for multi-spectral optical axis testing, we adopt electronic beaming vacuum depositing method with the aid of ion assistant deposition systems. Through analyzing the optical characters of coating material, film designing, monitoring thickness error, and optimizing technical parameters, we deposit multi-band laser filter film on the substrate of ZnS to achieve beam split of multi-band spectrum. In condition of 30° incident angle on both coating surface, the average transmittance exceeds 90% through 400～700 nm wavelength. Moreover, it makes the transmittance over 92% in 10.6 μm, below 5% in wavelength 1064 nm and 1540 nm. In addition, our experiment improves the adhesion of the film. It can resist laser beams and conform to environmental stability standards, completely meet the demands of optical instruments.