The calibration of structured light plane is the key technique in structured light three-dimensional vision measurement. The traditional methods require expensive equipments and complex procedure, and the efficiency is quite low. A novel method for structured light plane calibration is presented. In this method, a simple two-dimensional planar target is used. The target can be freely moved to different positions in the visible space of the camera if only the target and the structured light plane can intersect to each other. The corresponding world coordinate is set for each target in different positions. Transformation relation among the world coordinate, camera coordinate and image coordinate can be calculated. Through processing, the intersecting lines′ equations of different positions are unified at the camera coordinate system. Then we can use least square method to fit the structured light plane′s equation. The experimental results show that the proposed method is a universal method with high efficiency and simple procedure.