The enhanced fluorescence effect of the heterojunction of CdSe quantum dots and a porous Al2O3 film was experimentally studied by using TiN nanoparticles (NPs). TiN NPs were deposited on the surface of porous Al2O3 film. Then, the CdSe QDs were self-assembled on the surface of TiN/Al2O3 film to prepare the CdSe/TiN/Al2O3 heterojunction. At the same time, the surface enhanced fluorescence effect was observed on the platform of a scanning near-field optical microscope. The results have showed that the interfacial fluorescence from the porous Al2O3 film was enhanced due to the increase of poto-generated carriers resulted in by TiN Nps from the CdSe QDs to the porous Al2O3 film. These results based on this paper could be widely applied for many fields of photovoltaic, lightshows, optical sensors devices and nano-biological imaging system.