Herein, a novel method for generating parallelized fluorescence depletion patterns based on optical wedges is proposed. Optical wedges and matching reflectors are used to control the stimulated emission depletion (STED) beam inclination angle of the tube lens object space to make full use of the numerical aperture of the microscope objective to produce parallelized fluorescence depletion patterns with small periodicity. Simulation results show that a square lattice-like parallelized fluorescence depletion pattern with periodicity as small as 282.0 nm×283.6 nm is generated when the wavelength of the STED beam is 760 nm and numerical aperture of the microscope objective is 1.4, thereby achieving relatively high imaging resolution.