The effects of multiple influencing factors on soybean urease-induced calcium carbonate precipitation (SICP) were explored to select the main influencing factors and provided their optimal ranges. Firstly, the influence of urease concentration and temperature on urease activity were analyzed. After that, based on orthogonal test design, 25 test conditions of SICP aqueous solution tests were conducted, and the variation law of Ca2+ utilization ratio was studied through the combination of different factors. Also the morphology of CaCO3 was observed with SEM in diverse test conditions. The results show that low temperature delivers benefits to urease preservation and its activity exertion, urease activity can be preserved over 21 d with 5 ℃. At the same temperature, the greater the urease concentration is, the higher the initial activity of urease boasts and the shorter the time required for complete inactivation of urease. The pH value and the volume ratio of urease to cementation solution are the main factors affecting the Ca2+ utilization ratio. In order to achieve a higher Ca2+ utilization ratio, the optimum volume ratio of urease to cementation solution could be 1 while the optimal concentration ratio of CaCl2 to urea is 1.5, and the best Ca2+ concentration is 1 mol/L. More hexahedral CaCO3 is produced, when the urease concentration is low, and the shape of precipitated CaCO3 turns into spherical as the urease concentration increases. Aspartic acid which is rich in soybeans, is an significant factor in controlling the form of CaCO3.