In view of the lack of effective training means for aircraft knowledge, and the problem about the imbalance and deficiency between the knowledge of aircraft system and aircraft performance happend to the students majoring in traffic management. Base on working ability demand, a civil aircraft knowledge assistant training system is developed for senior undergraduates. The system is divided into two modules: aircraft system and aircraft performance, which integrates autonomous learning, testing and training, assessment and evaluation, and it can meet the training goal of traffic management students on aircraft system and aircraft performance knowledge. In addition, the software adds the “characteristic learning” module of domestic civil aircraft. Through the training of this system, students can choose their own question bank, set the topic question independently, and systematicly and actively learn the aircraft knowledge, which improves the ability of mastering aircraft theory.