Human myeloid differentiation factor 88 (MyD88) is the central factor for inflammatory signaling pathways downstream of members of the Toll-like receptor (TLR) and interleukin-1 (IL-1) receptor families. To explore the human MyD88 structures and functions by bioinformatics analysis, results showed human MyD88 gene of full length 5 670 bp, located on chromosome 3p22.2, and encoded 296 amino acids, which was a higher conserved one. Meanwhile, human MyD88 was highly distributed in the blood, spleen and lung, with no signal peptides, whereas it included 2 sumolation sites and 15 serine phosphorylation sites, 6 threonine phosphorylation sites and 4 tyrosine phosphorylation sites. The secondary structure was mainly consisted of 145 α-helix and 30 β-sheet regions, indicating that the tertiary structure was reliable by ramachandran plot. In addition, human MyD88 also has protein interaction with Toll-like receptors and interleukins, participating in inflammatory reaction, cell apoptosis and other immunologic processes. All together, our bioinformatics analysis would provide a basis for understanding structure features and biological functions of human MyD88, guiding a new schema for the diagnosis and treatment in the future.