Utilization of nonbonding conformational locks （NCLs） to enhance the electron mobility of solar cell materials and the photovoltaic conversion efficiency of the devices has been widely accepted. Currently， studies on the working mechanism of NCLs were mainly based on macroscopic experiments， while the regulation of charge conduction mechanism by NCLs at single-molecule level was rarely researched. In this paper， single-molecule conductance measurements of organic conjugated molecular systems containing NCLs were performed using scanning tunneling microscopy （STM） to investigate the effect of NCLs on intramolecular charge transport at the single-molecule level. It showed that NCLs could promote intramolecular charge conduction by planarizing the molecular conformation and modulating the molecular band gap， while DFT calculations could confirm that NCLs could furtherly enhance intramolecular charge conduction by establishing additional tunneling channels. Therefore， the results of this study provided a theoretical and experimental foundation for the design of high-efficiency organic photovoltaic materials based on NCLs.