Objective to determine the effect of transmyocardial laser revascularization (TMLR) in combined with the implantation of endothelial progenitor cells (EPCs) on cardiac function and neovascularization of myocardial ischemia. An operation of acute myocardial ischemia was created. Afterwards, the dogs were randomized into AMI group, TMLR group and TMLR+EPCs receiving TMLR with implantation of EPCs and calcium alginate into channels induced by laser. Four weeks later, all dogs underwent echocardiography and the samples of myocardial ischemia tissue underwent histochemical and immunohistologic analysis. The results show that TMLR+EPCs group regional contractility in the left artial diameter (LAD) territory revealed an improvement versus control and TMLR groups, whereas control and TMLR animals did not show any difference. Neoangiogenesis was observed in laser channels in the canine heart subjected to TMLR+EPCs treatment, the remains of clacium alginate appeared in the channels of TMLR+EPCs group, but the fibrosis in TMLR group. The result indicates TMLR in combined with EPCs improve the cardiac regional and global function in acute myocardial ischemia from advancing impariment, and augment neovascularization in channels of ischemic myocardium induced by laser.