The LIF spectrum of A2+(v=0) level of NO molecule has been studied by one-photon excitation. Twelve vibrational bands of A2+(v= 0-11) transition were observed. By using the method of two-step excitation, we studied for the first time the LIF spectrum in UV region after the E2+(v=0) energy level was excited. The vibrational bands directly from E2+(v=0) level to ground state X2H were obviously measured.