Nuclear magnetic resonance spectrometer is one of the most powerful tools for qualitative analysis of the composition and structure of various organic and inorganic substances. Therefore, nuclear magnetic resonance spectrometer is of great significance in the fields of organic chemistry, pharmaceutical engineering and medicine. At present, Bruker is still the mainstream of nuclear magnetic resonance spectrometer in China, and the market share of nuclear magnetic resonance spectrometer of JEOL is not high. In recent years, due to trade frictions between China and the United States, Japan’s electronic nuclear magnetic resonance spectrometer has gradually increased in the domestic market. However, the corresponding use and maintenance experience is still insufficient. Combined with the actual use of JNM-ECZ400S/L1 nuclear magnetic spectrometer in our college, this paper summarizes the management, maintenance and daily fault solutions of JEOL nuclear magnetic equipment, so as to provide reference experience for peers.