The protection of urban biodiversity is increasingly important in the context of the construction of Beautiful China and Ecological Civilization. Currently, there are deficiencies in biodiversity conservation objectives, values and strategies in territorial spatial planning. In order to further strengthen the connection between biodiversity and territorial spatial planning, and to provide planning response ideas, this paper analyzes the hierarchy and scale of biodiversity, as well as the current research on the impact of urban planning on biodiversity in China. It further explores the main sectors and key elements of territorial spatial planning impact on urban biodiversity, and then proposes urban territorial spatial planning strategies for biodiversity conservation, including developing a strategic framework, and proposing planning strategies at the general level and the detailed level, with a view to providing a reference for the work on biodiversity protection in territorial spatial planning.