Compared the conventional cleaning methods (chemical cleaning, ultrasonic cleaning), laser cleaning is recognized as a effective cleaning method without damage, precise removal, avoiding environments pollution and it is widely studied and used. According to the different mechanisms, laser cleaning can be classified as dry laser cleaning, steam laser cleaning and laser plasma shockwave. In the fields of cultural relics protection, the diseases on the surface of stone relics will affect the conservation and artistic quality of relics. In the high-power laser facility, contaminations on the surface of optics seriously limit normal operation of the facilities. Laser cleaning is used for removal the ink mark contamination on the surface of sandstone and the particles and grease contamination on the surface of gold-coated K9 glass. The laser cleaning effect has been characterized by optical microscopy, dark-field imaging, ultraviolet-visible-near infrared spectroscopy, X-ray photoelectron spectroscopy. The results indicated the cleaning effect is obvious and effective.