In recent years, infrared spectroscopy technology has been rapidly developed and widely used in forensic science. However, data analysis is complicated, and traditional algorithms are no longer applicable. Therefore, it is necessary to combine chemometrics to improve the accuracy and scientificity of material evidence analysis. In this paper, infrared spectroscopy and chemometrics are introduced and summarized in detail, and the application progress of the combination of the two in the field of trace evidence, food and drug ring evidence, biological evidence and document material evidence analysis is reviewed. At the same time, its future development direction and application prospect are also discussed.