A miniaturized, low-cost, and portable cell microscopic image acquisition system designed for wireless transmission has been developed, using Unigraphics NX software and three-dimensional printing for system structure to achieve cost efficiency and lightweight. The system measures 13 cm×5 cm×20 cm and weighs ~1.5 kg. An intelligent microimage acquisition software platform is built using functions from the OpenCV library. Testing results demonstrate that the proposed system successfully transmits microscopic images wirelessly via WiFi, performs cell counting and cell migration, and meets the requirements of standard cell observation experiments. The resolution of system is 1.5 μm, which is suitable for both teaching and scientific research applications.