To cultivate high quality talents with horticultural professional skills, a comprehensive assessment model covering skills mastery, practical application, team collaboration, and other aspects in the practical teaching was designed of Horticultural professional skills training course, and was put it into practice. In terms of program design, taking Horticulture professional skills training Ⅱ as an example, knowledge objectives, ability objectives, and quality objectives were determined, and the entire process assessment was divided into 4 stages: theoretical knowledge assessment (20%), practical operation assessment (35%), classroom performance assessment (20%), and report presentation assessment (25%). In terms of practice, guide students in the entire process of cultivation management from “seeds” to “fruits”, including tray planting, potted planting, cucumber and tomato planting, etc., comprehensively evaluating students’ learning process, and cultivating their practical operation, problem-solving, and innovation abilities. Practice has shown that the whole process assessment effectively enhances students’ learning enthusiasm and initiative, and the excellent performance rate of students has been increasing year by year, from 73.33% in 2022 to 100.00% in 2024. This article provides a reference for improving the effectiveness of teaching reform in similar courses and enhancing students’ practical abilities.