Objective: the effect of low-level laser on Canine distal movement was evaluated to evaluate the efficiency of low-level laser on accelerating orthodontic tooth movement. Methods: this meta-analysis follows the PRISMA statement. We searched PubMed, Cochrane Library, Embase, CNKI, VIP, and WanFang, and the search was conducted between January 2000 and December 2021. We pooled all randomized controlled trials and conducted a meta-analysis using Revman 5.3 and STATE. Results: a total of 224 samples were included in 7 studies. The results of meta-analysis show that: compared with the control group, the low-level Laser group within 7 days after treatment (WMD = 0.360, 95% CI = 0.330-0.389, P < 0.05) ; Within 28 days (WMD = 0.353, 95% CI = 0.141-0.566, P < 0.05) , within 60 days (WMD = 0.301, 95% CI = 0.101-0.502, P < 0.05) , within 90 days (WMD = 0.414, 95% CI = 0.179-0.650, P< 0.05) , the canine movement distance increased significantly. Conclusion: as an auxiliary means of orthodontic treatment, Low-level laser can effectively accelerate orthodontic tooth movement.