Carbon nanotubes (CNTs) were prepared by CH4 pyrolysis using chemical vapor deposition (CVD) technique. And then spinning disc processor (SDP) self-assembly technique was used to modify the CNTs with nano Pt and nano Au through electrodeposition processes. Subsequently, CNTs-Pt/GCE electrode and CNTs-Au modified electrode were prepared by electrochemical deposition of platinum particles on CNTs-modified glassy carbon electrode (GCE) and immobilization of glucose oxidase on Au electrode via electrostatic effect, respectively. Finally, the electrochemical behavior of glucose on CNTs-Au electrode and formaldehyde on CNTs-Pt/GCE electrode was investigated by cyclic voltammetry (CV) method in a three-electrode system. The electrochemical test results show that Au nanoparticles and CNTs could effectively increase the specific surface area of the electrode and increase its electron transfer rate. Therefore, CNTs-Au biosensor has good repeatability, stability and rapid glucose detection response, which could be used for the detection of serum glucose concentration in clinical practice. In addition, due to the high dispersion of Pt nano particles and the synergistic effect of CNTs and Pt nano particles, the active sites on the electrode surface are enhanced, so the CNTs-Pt/GCE electrode exhibits high sensitivity and good reproducibility in the detection process of formaldehyde. The above results shows that this work has a very good potential for practical application.