Regional culture is the representative of excellent regional culture with unique historical, humanistic and aesthetic values. Designers ingeniously integrate the regional culture with rich connotation into tourism clothing products to effectively promote the inheritance of regional culture and drive the development of the tourism cultural and creative industry. Starting from the value of applying regional culture in the design of tourism clothing, this paper analyzes the integration and inheritance status of regional culture in the design of tourism clothing products, and then explores the reasonable infiltration of regional culture in tourism clothing from professional talent cultivation, cultural connotation exploration, product differentiation design, brand construction and other aspects, so as to provide support for the live transmission and development of regional culture.