In order to study the interference effect of multispectral CCD camera, both xenon lamp (white light) and laser with wavelength of 671nm, 473nm, and 532nm separatelywere used to interfere the multispectral CCD camera.The interference map of red, green and blue channels was extracted, and the interference effect was analyzed. Finally, the white light irradiation multispectral CCD was simulated by using the diffusion model of photo generated carriers. The results show that red, green, and blue channels will be interfered at the same time when white light irradiates the multispectral CCD camera, and the interference effect is obviously better than that of single wavelength.When the incident power of white light is 10.5μW, the saturation pixel number of white light irradiated multispectral CCD is 2382pixel.With the increase of incident power, the number of saturated pixels of multispectral CCD increases gradually.When the incident power of white light is 980μW, the saturation pixel number of white light irradiated multispectral CCD is stable at 320078pixel. The order of the response of multispectral CCD tosuccessive laser is white light, 532nm, 473nm, and 671nm.The simulated interference figure and the curve of the number of saturated pixels changing with the laser power are basically consistent with the experiment.The results are helpful to further study the interference mechanism of multispectral CCD camera.