In order to reveal the characteristics of ectomycorrhizal fungi EMF diversity of Pinus armandii in subtropical karst mountain typical P armandii plantations in Central Guizhou were selected as the investigation sites and the EMF associated with P armandii was analyzed by combining morphological and molecular biology techniques The results showed that a total of 21 EMF OTUs were identified belonging to 12 families and 12 genera EMF OTUs richness of Basidiomycetes and Ascomycetes were 80 95% and 19 05% respectively indicating that Basidiomycetes were significantly more abundant than Ascomycetes The dominant groups of EMF community in P armandii were Thelephoraceae and Sebacinaceae Soil pH and total phosphorus were important factors affecting EMF community of P armandii