Two cases of three-banded pattern were detected with their saliva spots and hairs by Goldeneye 20A kit, PowerPlex 6C, Investigator 24plex QS and Huaxia Platinum kit. One case of tri-allele in children in D18S51(15/16/17) and the type of this loci of the children’s mother is 13 and 16, another case of tri-allele in father in D7S820(8/11/12) and the child is 10 and 11 were genotyped with Goldeneye 20A kit. The results of saliva spot and hair samples are consistent with those of FTA blood card.The results were the same as those by PowerPlex 6C, Investigator 24plex QS and Huaxia Platinum kit. More attention should be paid to the paternity testing because of the rare three-banded patterns of STR loci.