In order to meet the requirements of sustainable growth and development for human beings, biomedical research has always been our focus. Terahertz(THz)has been widely used in biomedicine because of its own advantages. This article presents the applications of THz characterization techniques and THz biological effects in biomedicine. The applications of THz characterization technology are divided into five parts: amino acid and polypeptide, DNA, protein, the detection of cancer and other applications such as caries detection. The researches of THz biological effects mainly concentrate on the effects of THz on the tissues and cells. Furthermore, this article briefly introduces chemometrics method and its applications in biomedical applications in combination with THz technology. In this review, we summarize the drawbacks of THz technology in biomedical field and discuss the direction of further research. This article reviews the applications of THz technology in biomedicine, and establishes the foundation for relevant study.