In the application of pulse power technology in tumor ablation, sewage treatment and other fields, it is found that bipolar electric pulse is often better than unipolar electric pulse, which greatly stimulates the research and development demand of bipolar high-voltage pulse power supply. A constant peak bipolar pulse generator is designed based on Boost closed-loop control. The generator perfectly combines the characteristics of Boost circuit and Marx generator to realize the generation of bipolar pulse with boost function. The peak detection circuit is used to sample the peak value of bipolar pulse and feed it back to DSP to realize closed-loop control, so as to realize the output of constant peak bipolar pulse. To verify the feasibility and stability of the proposed topology circuit, simulation and experiment of the developed 5-stage constant peak bipolar pulse generator are carried out. The experimental results show that when the input voltage is 100 V, a constant peak bipolar pulse waveform with repetition frequency of 5 kHz, pulse width of 5-10 μs and voltage amplitude of ±2.0 kV can be generated. The pulse power supply uses modular design, which is easy to cascade, compact, and can flexibly output bipolar or unipolar positive (negative) pulses with constant peak value.