The application of 5G communication technology and Internet of Things (IoT) technology, their applications in the field of education have become increasingly widespread, providing strong technical support for the modernization and transformation of disciplines. Utilizing the ultra-high speed, ultra-low latency and massive connectivity capabilities of 5G, combined with the intelligent sensing and interconnection characteristics of IoT technology, an efficient, intelligent and safe smart laboratory for optometry technology was designed and realized, and its system architecture, key technologies, realization path and application effects were described.Teaching practice showed that the smart laboratory improved the teaching quality and training effect, promoted the optimal allocation and sharing of educational resources, provided an innovative platform for the cultivation of optometry technology talents.