PhotoniX, Volume. 5, Issue 1, 7(2024)

Visualizing cortical blood perfusion after photothrombotic stroke in vivo by needle-shaped beam optical coherence tomography angiography

Xiangyu Guo1、†, Jingjing Zhao2,9、†, Liqun Sun1, Varun Gupta3, Lin Du5, Komal Sharma2, Aidan Vleck2, Kaitlyn Liang4, Liangcai Cao1, Lingjie Kong1, Yuanmu Yang1, Yong Huang6、*, Adam Zerda2,7,8、**, and Guofan Jin1、***
Author Affiliations
  • 1State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University, Beijing 100084, China
  • 2Department of Structural Biology, Stanford University School of Medicine, Stanford University, Stanford, CA 94305, USA
  • 3Department of Neurosurgery, Stanford University School of Medicine, Stanford University, Stanford, CA 94305, USA
  • 4Department of Radiology, Stanford University School of Medicine, Stanford University, Stanford, CA 94305, USA
  • 5Department of Electrical Engineering and Computer Sciences, University of California, Berkeley 94720, USA
  • 6School of Optics and Photonics, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Haidian, Beijing 100081, China
  • 7Biophysics Program, Molecular Imaging Program, and Bio-X Program at Stanford University, Stanford, CA 94305, USA
  • 8Chan Zuckerberg Biohub, San Francisco, CA 94110, USA
  • 9Institute of Medical Equipment Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China
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    Optical imaging techniques provide low-cost, non-radiative images with high spatiotemporal resolution, making them advantageous for long-term dynamic observation of blood perfusion in stroke research and other brain studies compared to non-optical methods. However, high-resolution imaging in optical microscopy fundamentally requires a tight optical focus, and thus a limited depth of field (DOF). Consequently, large-scale, non-stitched, high-resolution images of curved surfaces, like brains, are difficult to acquire without z-axis scanning. To overcome this limitation, we developed a needle-shaped beam optical coherence tomography angiography (NB-OCTA) system, and for the first time, achieved a volumetric resolution of less than 8 μm in a non-stitched volume space of 6.4 mm × 4 mm × 620 μm in vivo. This system captures the distribution of blood vessels at 3.4-times larger depths than normal OCTA equipped with a Gaussian beam (GB-OCTA). We then employed NB-OCTA to perform long-term observation of cortical blood perfusion after stroke in vivo, and quantitatively analyzed the vessel area density (VAD) and the diameters of representative vessels in different regions over 10 days, revealing different spatiotemporal dynamics in the acute, sub-acute and chronic phase of post-ischemic revascularization. Benefiting from our NB-OCTA, we revealed that the recovery process is not only the result of spontaneous reperfusion, but also the formation of new vessels. This study provides visual and mechanistic insights into strokes and helps to deepen our understanding of the spontaneous response of brain after stroke.

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    Xiangyu Guo, Jingjing Zhao, Liqun Sun, Varun Gupta, Lin Du, Komal Sharma, Aidan Vleck, Kaitlyn Liang, Liangcai Cao, Lingjie Kong, Yuanmu Yang, Yong Huang, Adam Zerda, Guofan Jin. Visualizing cortical blood perfusion after photothrombotic stroke in vivo by needle-shaped beam optical coherence tomography angiography[J]. PhotoniX, 2024, 5(1): 7

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    Paper Information

    Category: Research Articles

    Received: Nov. 8, 2023

    Accepted: Mar. 13, 2024

    Published Online: Apr. 9, 2024

    The Author Email: Huang Yong (huangyong2015@bit.edu.cn), Zerda Adam (adlz@stanford.edu), Jin Guofan (jgf-dpi@mail.tsinghua.edu.cn)

    DOI:10.1186/s43074-024-00124-9

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