Based on the diffraction wavelength selectivity of reflection holography and the colorimetric principle, the expression of reflection hologram colorimetric system is given, and the conversion relationship between reflection hologram colorimetric system and CIE XYZ system is studied. The factors influenced color of reflection hologram are analyzed and discussed, and the color evaluation method between real object and reconstructed image of reflection hologram is proposed. For various color samples, the chromatism of reflection holograms under different trichromatic systems is calculated by the conversion relations of trichromatic system and chromatic evaluation，and the color difference analysis is carried on.