When the transverse force was measured by using fiber Bragg grating (FBG),some parameters,hard to be measured,influenced severely the reflective spectral model of FBG. Therefore,ant colony algorithm was proposed in analyzing the traverse uniform strain reflective spectral model of FBG. Base on the traverse uniform strain sense theory of FBG and ants algorithm,the objective function in analyzing traverse uniform strain reflective spectrum of FBG is established,meanwhile corresponding experimental system is constructed. Aiming at the parameters combination from the experimental results,traverse loaded reflective spectrum of FBG is reconstructed using the ant colony algorithm. Experimental data showed that ant colony algorithm used in analyzing of traverse uniform strain reflective spectrum expression of FBG,had some advantages,such as high accuracy,stability and fast convergence rate. And some main parameters influenced the reflective spectrum could obtained easily.