Laser & Optoelectronics Progress, Volume. 62, Issue 18, 1817004(2025)

Advance in Fourier Light-Field Microscopy and Its Biological Application (Invited)

Qianbo Liu1, Deer Su1, Youming Xiao2, Zonglin Guo2, Jin Wu2, Jian Wang2, Changliang Guo3、***, Haoyu Li1、**, and Wenhao Liu1、*
Author Affiliations
  • 1School of Instrumentation Science and Engineering, Harbin Institute of Technology, Harbin 150080, Heilongjiang , China
  • 2School of Physics, Harbin Institute of Technology, Harbin 150080, Heilongjiang , China
  • 3College of Future Technology, Peking University, Beijing 100871, China
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    Light-field microscopy has demonstrated remarkable potential in biomedical imaging. However, its inherent challenges—such as uneven spatial sampling and reconstruction artifacts—have limited its broader application. To address these issues, this paper introduces the recent advancements in Fourier light-field microscopy (FLFM). By recording light field information in the Fourier domain, FLFM achieves a consistent aliasing of both spatial and angular components of incident light. This innovative approach effectively mitigates reconstruction artifacts and enhances the uniformity of imaging resolution. In this work, we provide a comprehensive explanation of the imaging principles, system designs, and reconstruction algorithms of FLFM, comparing its point spread function to that of conventional light-field microscopy. Furthermore, we explore the application of FLFM in biological research, particularly in subcellular imaging and model organism studies. Finally, we offer insights into the future directions of FLFM.

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    Qianbo Liu, Deer Su, Youming Xiao, Zonglin Guo, Jin Wu, Jian Wang, Changliang Guo, Haoyu Li, Wenhao Liu. Advance in Fourier Light-Field Microscopy and Its Biological Application (Invited)[J]. Laser & Optoelectronics Progress, 2025, 62(18): 1817004

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    Paper Information

    Category: Medical Optics and Biotechnology

    Received: May. 27, 2025

    Accepted: Jun. 19, 2025

    Published Online: Sep. 16, 2025

    The Author Email: Changliang Guo (changliangguo@pku.edu.cn), Haoyu Li (lihaoyu@hit.edu.cn), Wenhao Liu (serenewelkin@qq.com)

    DOI:10.3788/LOP251327

    CSTR:32186.14.LOP251327

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