The silicate glass doped with CdTe quantum dots is prepared by the high temperature melt-quenching technique and the Raman, absorption and emission spectra are tested. The quantum size effect of quantum dot doped glass is verified. The up-conversion fluorescence of the CdTe quantum dot doped glass is excited by 800 nm and 960 nm femtosecond lasers, which is confirmed to be two-photon absorption induced luminescence and the two-photon fluorescence possesses a requirement for a certain range of wavelengths. The nonlinear absorption coefficient of the CdTe quantum dot doped glass is obtained up to 3.62×10-11 m·W-1.