To establish a method for the determination of lithium, boron, sodium, magnesium, aluminum, potassium, calcium, vanadium, chromium, manganese, iron, cobalt, nickel, copper, zinc, arsenic, selenium, rubidium, strontium, molybdenum, cadmium, tin, antimony, barium, mercury and lead in Armillaria luteo-virensby microwave digestion (MWD) and inductively coupled plasma mass spectrometry (ICP-MS). 8 mL of nitric acid was added as the digestion solution, and after digesting A. luteo-virens by MWD, the volume was made up to 50 mL, and 26 elements were determined by ICP-MS. On the basis of optimizing the parameters of dilution ratio, carrier gas and diluent gas ratio, the correlation coefficients of the obtained element curves were all above 0.999 2 except boron. The detection limit of the method was 0. 002~0. 540 μg·L^-1, and the coefficient of variation in batch was 0. 69%~7. 69%. The coefficient of variation between batches was 1. 13%~10. 61%, and the recovery rate of low concentration plus standard was 80. 1%~145. 2%. The recoveries were 88. 9%~131. 2%. The recoveries of high concentration plus standard were 82. 4%~118. 2%. The quality control samples of 26 metals in edible fungi were in accord with the reference values. The method has the advantages of high accuracy, low detection limit and wide linear range, the precision and accuracy meet the detection requirements. It is applicable to the rapid and simultaneous determination of 26 elements in the local specialty food A. luteo-virens.