A scheme on frequency up-conversion of radio-over-fiber (RoF) signal is proposed to use dispersion-compressed short pulse rather than the tradition continuous wave carrier as carrier. By compressing pulse width based on optical fiber dispersion, harmonic component is strengthened, signal component over pulse carrier at the receiving end is converted to higher harmonic waves, and finally the up-conversion is completed at the remote antenna unit (RAU). The generation of high-frequency microwave signal with low frequency local oscillator (LO) doesn′t need any electric frequency multiplier and mixer in transmitting terminal, rather than a filter which can select the desired signal in receiving terminal, and the system is simplified. In this system only with an optical amplifier, 2 Gbit/s signal can be up-converted to 16 GHz after transmission of 60 km standard single-mode fiber (SMF), and the bit error rate (BER) is below 10-8 after 5.4 m wireless communication, no need of on-line, pre-amplification, dispersion compensation and optical filter.