Associated liquids is a kind of common media in daily life and industrial production process, and their acoustic and viscoelastic properties are very important in many scenes. To this end, the Brillouin scattering spectroscopy is used to measure the acoustic and viscoelastic properties of two representative associative liquids, pure water and glycerol, at different temperatures. Complete spectra were obtained at 180° backscattering geometry. Based on a full theoretical description of the system, the acoustic absorption coefficient and shear viscosity were calculated, and the influence of temperature on those properties was further achieved.