Mechanism of chemotherapeutics for tumor therapy is to restore infinite proliferous cancer cells to normal apoptosis, therefore, apoptosis monitoring has important guidance for clinical treatment. A novel and robust method for apoptosis monitoring of tumor cells is engineered based on dynamic laser tweezers, using SKOV3 cell line as typical sample. SKOV3 cells are cultured under four gradient concentrations of cisplatin, and trapping efficiency of four groups are measured by proposed system, which presents great advantages of celerity, low consumption, and label-free explorations for living cells without perturbing experimental conditions in combination with classical probes. The proposed technique has great potential in improving cancer treatment by monitoring the objective efficacy of tumor cell killing.