To test the contribution of the direct effects of photodynamic therapy (PDT) on tumor cells, we examined the immunogenicity of PDT-generated murine tumor cell lysates in a preclinical vaccine model. Sixty Kunming mice (H22 tumor host) were divided into two groups randomly and equally. Six to twelve-week-old Kunming mice were vaccinated intradermally on the right shoulder with 50 μL lysates (3×105 cell equivalents) for experimental group or medium control for control group every three days during two weeks. The mice rested a week and then inoculated on the flank with 1×106 tumor cells harvested from exponentially growing cultures. And then we compared antitumor rate, survial rate and relevant indicators of immunology between two groups. PDT vaccines could inhibit the tumor growth rate compared to the contrast group. The tumor inhibition rate of PDT vaccines group was 60% and long-term available. The survival rate of PDT vaccines group at 100 day was 56% which was significantly higher than contrast group. Our studies suggest that PDT-generated vaccines could effectively inhibit tumor growth, improve survival rate of mice in experimental group, and enhance antitumor immune response significantly. PDT-generated vaccines may have well clinical potential as an adjuvant therapy.