Nd-doped phosphate glass photonic crystal fiber (PCF) with a 25 μm diameter core was prepared by the stack-and-draw method. Amplified spontaneous emission (ASE) spectra of the PCF were investigated by end pumping. The result showed that PCF has stronger peak at 1060 nm and dramatically weakened peaks at 900 nm and 1330 nm, while linewidth of the peak at 1060 nm gets narrower slightly than linewidth of glass rod. The single mode operation was obtained at 1060 nm with a measured loss of 9.1 dB/m. The potential advantages of application in the area of fiber laser were discussed.